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anti casr polyclonal antibody  (Proteintech)


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    Structured Review

    Proteintech anti casr polyclonal antibody
    Anti Casr Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+casr+antibody/Calcium+Sensing+Receptor+Antibody/pm41801666-90-19-23
    Average 93 stars, based on 34 article reviews
    anti casr polyclonal antibody - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Functional Assay:

    Article Title: Extracellular Ca 2+ promotes nitric oxide production via Ca 2+ -sensing receptor-G q/11 protein-endothelial nitric oxide synthase signaling in human vascular endothelial cells.
    Article Snippet: .. These results indicate that functional CaSR is expressed in EA.hy926 cells and Gq/11-dependently activated by its endogenous ligand Ca2þ when physiological concentrations of Ca2þ are between 1.0 and 1.3 mM.1 To confirm the expression of CaSR protein in EA.hy926 cells, endogenously and exogenously expressed CaSR was detected with an anti-CaSR antibody (Proteintech Group, Inc., Rosemont, IL, U.S.A.). ..

    Expressing:

    Article Title: Extracellular Ca 2+ promotes nitric oxide production via Ca 2+ -sensing receptor-G q/11 protein-endothelial nitric oxide synthase signaling in human vascular endothelial cells.
    Article Snippet: .. These results indicate that functional CaSR is expressed in EA.hy926 cells and Gq/11-dependently activated by its endogenous ligand Ca2þ when physiological concentrations of Ca2þ are between 1.0 and 1.3 mM.1 To confirm the expression of CaSR protein in EA.hy926 cells, endogenously and exogenously expressed CaSR was detected with an anti-CaSR antibody (Proteintech Group, Inc., Rosemont, IL, U.S.A.). ..

    Incubation:

    Article Title: Synergistic Effects of 25-Hydroxyvitamin D 3 , Phytase, and Probiotics on Growth, Calcium and Phosphorus Metabolism, and Bone Development in Weaned Piglets Fed Low Ca-P Diets
    Article Snippet: .. After transfer, the membranes were blocked with 5% ( w / v ) bovine serum albumin for 1 h at room temperature and then incubated overnight at 4 °C with the primary antibodies, including Anti-β-actin antibody (8115-1-RR, Proteintech, Rosemont, IL, USA, diluted 1:5000), Anti-CYP27B1 antibody (PA5-79128, Thermo Fisher Scientific, Waltham, MA, USA, diluted 1:2000), and Anti-CASR antibody (19125-1-AP, Proteintech, diluted 1:1000). .. Following three washes with TBST, the membranes were incubated with a rabbit recombinant secondary antibody (RGAR001, Proteintech) diluted 1:5000 at room temperature.

    Article Title: Synergistic Effects of 25-Hydroxyvitamin D 3 , Phytase, and Probiotics on Growth, Calcium and Phosphorus Metabolism, and Bone Development in Weaned Piglets Fed Low Ca-P Diets.
    Article Snippet: .. After transfer, the membranes were blocked with 5% (w/v) bovine serum albumin for 1 h at room temperature and then incubated overnight at 4 ◦C with the primary antibodies, including Anti-β-actin antibody (8115-1-RR, Proteintech, Rosemont, IL, USA, diluted 1:5000), AntiCYP27B1 antibody (PA5-79128, Thermo Fisher Scientific, Waltham, MA, USA, diluted 1:2000), and Anti-CASR antibody (19125-1-AP, Proteintech, diluted 1:1000). .. Following three washes with TBST, the membranes were incubated with a rabbit recombinant secondary antibody (RGAR001, Proteintech) diluted 1:5000 at room temperature.

    Article Title: Acquired hypocalciuric hypercalcemia in a dog
    Article Snippet: .. After blocking, the membranes were incubated with a primary antibody, an anti-CaSR antibody (19125-1; proteintech, Tokyo, Japan) used at a 1:500 dilution, for 1 h at RT, and then with a secondary antibody, an HRP-conjugated anti-rabbit IgG (sc-2004; Santa Cruz, Dallas, TX, USA) used at a 1:5000 dilution, for 1 h at RT. .. Protein bands were visualized using a chemiluminescence detection kit (Western BLoT Quant HRP Substrate; Takara Bio, Shiga, Japan).

    Blocking Assay:

    Article Title: Acquired hypocalciuric hypercalcemia in a dog
    Article Snippet: .. After blocking, the membranes were incubated with a primary antibody, an anti-CaSR antibody (19125-1; proteintech, Tokyo, Japan) used at a 1:500 dilution, for 1 h at RT, and then with a secondary antibody, an HRP-conjugated anti-rabbit IgG (sc-2004; Santa Cruz, Dallas, TX, USA) used at a 1:5000 dilution, for 1 h at RT. .. Protein bands were visualized using a chemiluminescence detection kit (Western BLoT Quant HRP Substrate; Takara Bio, Shiga, Japan).



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    Calcium oxalate crystals upregulate <t>CaSR</t> and SLC26A6 expression in vivo and in vitro . (A) Representative images of renal tissue pathological sections from each group of rats (HE staining, Pizzolato’s staining, and polarized light microscopy). (B) Western blotting detection of CaSR and SLC26A6 protein expression levels in NRK-52E cells after COM crystal intervention. (C,D) RT-qPCR (C) and Western blotting (D) detection of CaSR and SLC26A6 mRNA and protein expression levels in renal tissues of each group of rats. (E) Immunohistochemical analysis revealed the tubular localization patterns of CaSR and SLC26A6 in rat kidney tissue. Low-power field images demonstrated their widespread distribution within both the renal cortex and medulla. Precise cellular colocalization could only be assessed through high-power confocal analysis combined with segment-specific markers, representing a limitation of this study. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. NC group or Control group.
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    Calcium oxalate crystals upregulate <t>CaSR</t> and SLC26A6 expression in vivo and in vitro . (A) Representative images of renal tissue pathological sections from each group of rats (HE staining, Pizzolato’s staining, and polarized light microscopy). (B) Western blotting detection of CaSR and SLC26A6 protein expression levels in NRK-52E cells after COM crystal intervention. (C,D) RT-qPCR (C) and Western blotting (D) detection of CaSR and SLC26A6 mRNA and protein expression levels in renal tissues of each group of rats. (E) Immunohistochemical analysis revealed the tubular localization patterns of CaSR and SLC26A6 in rat kidney tissue. Low-power field images demonstrated their widespread distribution within both the renal cortex and medulla. Precise cellular colocalization could only be assessed through high-power confocal analysis combined with segment-specific markers, representing a limitation of this study. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. NC group or Control group.
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    Calcium oxalate crystals upregulate <t>CaSR</t> and SLC26A6 expression in vivo and in vitro . (A) Representative images of renal tissue pathological sections from each group of rats (HE staining, Pizzolato’s staining, and polarized light microscopy). (B) Western blotting detection of CaSR and SLC26A6 protein expression levels in NRK-52E cells after COM crystal intervention. (C,D) RT-qPCR (C) and Western blotting (D) detection of CaSR and SLC26A6 mRNA and protein expression levels in renal tissues of each group of rats. (E) Immunohistochemical analysis revealed the tubular localization patterns of CaSR and SLC26A6 in rat kidney tissue. Low-power field images demonstrated their widespread distribution within both the renal cortex and medulla. Precise cellular colocalization could only be assessed through high-power confocal analysis combined with segment-specific markers, representing a limitation of this study. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. NC group or Control group.
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    Proteintech anti casr antibody
    Calcium oxalate crystals upregulate <t>CaSR</t> and SLC26A6 expression in vivo and in vitro . (A) Representative images of renal tissue pathological sections from each group of rats (HE staining, Pizzolato’s staining, and polarized light microscopy). (B) Western blotting detection of CaSR and SLC26A6 protein expression levels in NRK-52E cells after COM crystal intervention. (C,D) RT-qPCR (C) and Western blotting (D) detection of CaSR and SLC26A6 mRNA and protein expression levels in renal tissues of each group of rats. (E) Immunohistochemical analysis revealed the tubular localization patterns of CaSR and SLC26A6 in rat kidney tissue. Low-power field images demonstrated their widespread distribution within both the renal cortex and medulla. Precise cellular colocalization could only be assessed through high-power confocal analysis combined with segment-specific markers, representing a limitation of this study. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. NC group or Control group.
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    Calcium oxalate crystals upregulate <t>CaSR</t> and SLC26A6 expression in vivo and in vitro . (A) Representative images of renal tissue pathological sections from each group of rats (HE staining, Pizzolato’s staining, and polarized light microscopy). (B) Western blotting detection of CaSR and SLC26A6 protein expression levels in NRK-52E cells after COM crystal intervention. (C,D) RT-qPCR (C) and Western blotting (D) detection of CaSR and SLC26A6 mRNA and protein expression levels in renal tissues of each group of rats. (E) Immunohistochemical analysis revealed the tubular localization patterns of CaSR and SLC26A6 in rat kidney tissue. Low-power field images demonstrated their widespread distribution within both the renal cortex and medulla. Precise cellular colocalization could only be assessed through high-power confocal analysis combined with segment-specific markers, representing a limitation of this study. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. NC group or Control group.
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    Calcium oxalate crystals upregulate <t>CaSR</t> and SLC26A6 expression in vivo and in vitro . (A) Representative images of renal tissue pathological sections from each group of rats (HE staining, Pizzolato’s staining, and polarized light microscopy). (B) Western blotting detection of CaSR and SLC26A6 protein expression levels in NRK-52E cells after COM crystal intervention. (C,D) RT-qPCR (C) and Western blotting (D) detection of CaSR and SLC26A6 mRNA and protein expression levels in renal tissues of each group of rats. (E) Immunohistochemical analysis revealed the tubular localization patterns of CaSR and SLC26A6 in rat kidney tissue. Low-power field images demonstrated their widespread distribution within both the renal cortex and medulla. Precise cellular colocalization could only be assessed through high-power confocal analysis combined with segment-specific markers, representing a limitation of this study. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. NC group or Control group.
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    ABclonal Biotechnology casr
    <t>CaSR</t> mediated the osteogenic‐promoting effects of L‐Trp on MC3T3‐E1 cells. (A) Expression levels of Casr and its downstream <t>genes</t> <t>(Gna11</t> and Pth1r). At 4 days, Casr expression was significantly higher in the L‐Trp‐treated group compared to the control group. By 7 days, the expression of Gna11 and Pth1r was also significantly elevated in the L‐Trp‐treated group. (B, C) Qualitative and semi‐quantitative analysis ( n = 3) of CASR‐related proteins. L‐Trp significantly upregulated the expression of CASR and GNA11. (D, E) Cell scratching experiment and the semi‐quantitative analysis. L‐Trp significantly enhanced the migration of MC3T3‐E1 cells. This effect was reversed by NPS‐2143, a CASR inhibitor, indicating that L‐Trp promoted cell migration through CASR activation. (F, G) ALP staining and alizarin red staining. L‐Trp significantly enhanced osteogenesis and the effect was attenuated by NPS‐2143, confirming that L‐Trp‐mediated osteogenic differentiation through CASR activation.
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    Image Search Results


    Calcium oxalate crystals upregulate CaSR and SLC26A6 expression in vivo and in vitro . (A) Representative images of renal tissue pathological sections from each group of rats (HE staining, Pizzolato’s staining, and polarized light microscopy). (B) Western blotting detection of CaSR and SLC26A6 protein expression levels in NRK-52E cells after COM crystal intervention. (C,D) RT-qPCR (C) and Western blotting (D) detection of CaSR and SLC26A6 mRNA and protein expression levels in renal tissues of each group of rats. (E) Immunohistochemical analysis revealed the tubular localization patterns of CaSR and SLC26A6 in rat kidney tissue. Low-power field images demonstrated their widespread distribution within both the renal cortex and medulla. Precise cellular colocalization could only be assessed through high-power confocal analysis combined with segment-specific markers, representing a limitation of this study. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. NC group or Control group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: CaSR regulates SLC26A6 expression via the PKA-FOXO4 signaling axis to promote experimental calcium oxalate kidney stone formation in rats

    doi: 10.3389/fcell.2026.1746423

    Figure Lengend Snippet: Calcium oxalate crystals upregulate CaSR and SLC26A6 expression in vivo and in vitro . (A) Representative images of renal tissue pathological sections from each group of rats (HE staining, Pizzolato’s staining, and polarized light microscopy). (B) Western blotting detection of CaSR and SLC26A6 protein expression levels in NRK-52E cells after COM crystal intervention. (C,D) RT-qPCR (C) and Western blotting (D) detection of CaSR and SLC26A6 mRNA and protein expression levels in renal tissues of each group of rats. (E) Immunohistochemical analysis revealed the tubular localization patterns of CaSR and SLC26A6 in rat kidney tissue. Low-power field images demonstrated their widespread distribution within both the renal cortex and medulla. Precise cellular colocalization could only be assessed through high-power confocal analysis combined with segment-specific markers, representing a limitation of this study. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. NC group or Control group.

    Article Snippet: Rabbit polyclonal antibodies against CaSR (73303S) and Phospho-PKA Substrate (9624S) were purchased from Cell Signaling Technology Company.

    Techniques: Expressing, In Vivo, In Vitro, Staining, Light Microscopy, Western Blot, Quantitative RT-PCR, Immunohistochemical staining, Standard Deviation, Control

    CaSR regulates SLC26A6 expression via the transcription factor FOXO4. (A) Western blotting detection of p-FOXO4 (Thr451) and SLC26A6 protein expression levels in NRK-52E cells treated with CaSR agonist (R568, 30 μM) or inhibitor (NPS-2143, 10 μM) in the presence of COM crystals. (B) Western blotting detection of SLC26A6 protein expression levels in NRK-52E cells treated with FOXO4 agonist (TNF-α, 20 ng/mL) or inhibitor (JY-2, 30 μM) in the presence of COM crystals. (C) Dual-luciferase reporter gene assay detecting the effect of FOXO4 on SLC26A6 promoter activity. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. COM group; #p < 0.05, ##p < 0.01 vs. specified group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: CaSR regulates SLC26A6 expression via the PKA-FOXO4 signaling axis to promote experimental calcium oxalate kidney stone formation in rats

    doi: 10.3389/fcell.2026.1746423

    Figure Lengend Snippet: CaSR regulates SLC26A6 expression via the transcription factor FOXO4. (A) Western blotting detection of p-FOXO4 (Thr451) and SLC26A6 protein expression levels in NRK-52E cells treated with CaSR agonist (R568, 30 μM) or inhibitor (NPS-2143, 10 μM) in the presence of COM crystals. (B) Western blotting detection of SLC26A6 protein expression levels in NRK-52E cells treated with FOXO4 agonist (TNF-α, 20 ng/mL) or inhibitor (JY-2, 30 μM) in the presence of COM crystals. (C) Dual-luciferase reporter gene assay detecting the effect of FOXO4 on SLC26A6 promoter activity. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. COM group; #p < 0.05, ##p < 0.01 vs. specified group.

    Article Snippet: Rabbit polyclonal antibodies against CaSR (73303S) and Phospho-PKA Substrate (9624S) were purchased from Cell Signaling Technology Company.

    Techniques: Expressing, Western Blot, Luciferase, Reporter Gene Assay, Activity Assay, Standard Deviation

    CaSR promotes FOXO4 phosphorylation via the PKA signaling pathway. (A) Western blotting detection of p-PKA substrate and p-FOXO4 (Thr451) protein expression levels in NRK-52E cells treated with CaSR agonist (R568, 30 μM) or inhibitor (NPS-2143, 10 μM) in the presence of COM crystals. (B) Western blotting detection of p-FOXO4 (Thr451) and SLC26A6 protein expression levels in NRK-52E cells treated with PKA agonist (8-Bromo-cAMP, 30 μM) or inhibitor (H-89 2HCl, 30 μM) in the presence of COM crystals. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. COM group; #p < 0.05, ##p < 0.01 vs. specified group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: CaSR regulates SLC26A6 expression via the PKA-FOXO4 signaling axis to promote experimental calcium oxalate kidney stone formation in rats

    doi: 10.3389/fcell.2026.1746423

    Figure Lengend Snippet: CaSR promotes FOXO4 phosphorylation via the PKA signaling pathway. (A) Western blotting detection of p-PKA substrate and p-FOXO4 (Thr451) protein expression levels in NRK-52E cells treated with CaSR agonist (R568, 30 μM) or inhibitor (NPS-2143, 10 μM) in the presence of COM crystals. (B) Western blotting detection of p-FOXO4 (Thr451) and SLC26A6 protein expression levels in NRK-52E cells treated with PKA agonist (8-Bromo-cAMP, 30 μM) or inhibitor (H-89 2HCl, 30 μM) in the presence of COM crystals. Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. COM group; #p < 0.05, ##p < 0.01 vs. specified group.

    Article Snippet: Rabbit polyclonal antibodies against CaSR (73303S) and Phospho-PKA Substrate (9624S) were purchased from Cell Signaling Technology Company.

    Techniques: Phospho-proteomics, Western Blot, Expressing, Standard Deviation

    Crystal deposition and tissue damage in the kidneys of rats from each group (A) Hematoxylin and eosin (HE) staining, polarized light microscopy, and Pizzolato’s calcium salt staining of kidney tissue from rats in the EG, CaSR-a, and CaSR-i groups. HE staining reveals tubular architecture and the extent of damage; polarized light microscopy reveals the birefringence of calcium oxalate crystals; Pizzolato’s staining stains calcium oxalate crystals black. (B) Hematoxylin and eosin (HE) staining, polarized light microscopy, and Pizzolato’s calcium salt staining of renal tissues from rats in the EG, PKA-i, and FOXO4-i groups. HE staining reveals the extent of tubular damage in each group; polarized light microscopy and calcium salt staining jointly confirm differences in crystal deposition among the groups.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: CaSR regulates SLC26A6 expression via the PKA-FOXO4 signaling axis to promote experimental calcium oxalate kidney stone formation in rats

    doi: 10.3389/fcell.2026.1746423

    Figure Lengend Snippet: Crystal deposition and tissue damage in the kidneys of rats from each group (A) Hematoxylin and eosin (HE) staining, polarized light microscopy, and Pizzolato’s calcium salt staining of kidney tissue from rats in the EG, CaSR-a, and CaSR-i groups. HE staining reveals tubular architecture and the extent of damage; polarized light microscopy reveals the birefringence of calcium oxalate crystals; Pizzolato’s staining stains calcium oxalate crystals black. (B) Hematoxylin and eosin (HE) staining, polarized light microscopy, and Pizzolato’s calcium salt staining of renal tissues from rats in the EG, PKA-i, and FOXO4-i groups. HE staining reveals the extent of tubular damage in each group; polarized light microscopy and calcium salt staining jointly confirm differences in crystal deposition among the groups.

    Article Snippet: Rabbit polyclonal antibodies against CaSR (73303S) and Phospho-PKA Substrate (9624S) were purchased from Cell Signaling Technology Company.

    Techniques: Staining, Light Microscopy

    Effects of intervening in the CaSR-PKA-FOXO4 signaling axis on urinary biochemical indicators and renal protein expression in rats. (A) Western blotting detection of p-PKA substrate, p-FOXO4 (Thr451), and SLC26A6 protein expression levels in renal tissues of each group of rats. (B) Concentrations of calcium ions and oxalate in 24-h urine of each group of rats. (C) Quantitative analysis of SLC26A6 protein expression from (A) . Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. E.G., group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: CaSR regulates SLC26A6 expression via the PKA-FOXO4 signaling axis to promote experimental calcium oxalate kidney stone formation in rats

    doi: 10.3389/fcell.2026.1746423

    Figure Lengend Snippet: Effects of intervening in the CaSR-PKA-FOXO4 signaling axis on urinary biochemical indicators and renal protein expression in rats. (A) Western blotting detection of p-PKA substrate, p-FOXO4 (Thr451), and SLC26A6 protein expression levels in renal tissues of each group of rats. (B) Concentrations of calcium ions and oxalate in 24-h urine of each group of rats. (C) Quantitative analysis of SLC26A6 protein expression from (A) . Data are presented as mean ± standard deviation, *p < 0.05, **p < 0.01 vs. E.G., group.

    Article Snippet: Rabbit polyclonal antibodies against CaSR (73303S) and Phospho-PKA Substrate (9624S) were purchased from Cell Signaling Technology Company.

    Techniques: Expressing, Western Blot, Standard Deviation

    Schematic diagram of the mechanism by which CaSR synergistically promotes calcium oxalate stone formation through dual signaling pathways. Under stimulation by calcium oxalate crystals (COM), the calcium-sensing receptor (CaSR) on the membrane of renal tubular epithelial cells is activated. The activated CaSR activates protein kinase A (PKA) via G proteins. PKA then functions through two parallel pathways: Phosphorylates Signal Transducer and Activator of Transcription 3 (STAT3), causing its nuclear translocation and upregulation of the tight junction protein Claudin-14 expression. Claudin-14 inhibits paracellular calcium reabsorption, leading to hypercalciuria; Phosphorylates Forkhead box protein O4 (FOXO4), causing its nuclear translocation and binding to the SLC26A6 gene promoter, upregulating its expression and promoting oxalate secretion into the lumen, leading to hyperoxaluria. The increased excretion of urinary calcium and oxalate collectively exacerbates the supersaturation of calcium oxalate in urine, ultimately promoting kidney stone formation.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: CaSR regulates SLC26A6 expression via the PKA-FOXO4 signaling axis to promote experimental calcium oxalate kidney stone formation in rats

    doi: 10.3389/fcell.2026.1746423

    Figure Lengend Snippet: Schematic diagram of the mechanism by which CaSR synergistically promotes calcium oxalate stone formation through dual signaling pathways. Under stimulation by calcium oxalate crystals (COM), the calcium-sensing receptor (CaSR) on the membrane of renal tubular epithelial cells is activated. The activated CaSR activates protein kinase A (PKA) via G proteins. PKA then functions through two parallel pathways: Phosphorylates Signal Transducer and Activator of Transcription 3 (STAT3), causing its nuclear translocation and upregulation of the tight junction protein Claudin-14 expression. Claudin-14 inhibits paracellular calcium reabsorption, leading to hypercalciuria; Phosphorylates Forkhead box protein O4 (FOXO4), causing its nuclear translocation and binding to the SLC26A6 gene promoter, upregulating its expression and promoting oxalate secretion into the lumen, leading to hyperoxaluria. The increased excretion of urinary calcium and oxalate collectively exacerbates the supersaturation of calcium oxalate in urine, ultimately promoting kidney stone formation.

    Article Snippet: Rabbit polyclonal antibodies against CaSR (73303S) and Phospho-PKA Substrate (9624S) were purchased from Cell Signaling Technology Company.

    Techniques: Protein-Protein interactions, Membrane, Translocation Assay, Expressing, Binding Assay

    CaSR mediated the osteogenic‐promoting effects of L‐Trp on MC3T3‐E1 cells. (A) Expression levels of Casr and its downstream genes (Gna11 and Pth1r). At 4 days, Casr expression was significantly higher in the L‐Trp‐treated group compared to the control group. By 7 days, the expression of Gna11 and Pth1r was also significantly elevated in the L‐Trp‐treated group. (B, C) Qualitative and semi‐quantitative analysis ( n = 3) of CASR‐related proteins. L‐Trp significantly upregulated the expression of CASR and GNA11. (D, E) Cell scratching experiment and the semi‐quantitative analysis. L‐Trp significantly enhanced the migration of MC3T3‐E1 cells. This effect was reversed by NPS‐2143, a CASR inhibitor, indicating that L‐Trp promoted cell migration through CASR activation. (F, G) ALP staining and alizarin red staining. L‐Trp significantly enhanced osteogenesis and the effect was attenuated by NPS‐2143, confirming that L‐Trp‐mediated osteogenic differentiation through CASR activation.

    Journal: FASEB BioAdvances

    Article Title: Levo‐Tryptophan Promotes Osteogenesis Through Calcium‐Sensing Receptor

    doi: 10.1096/fba.2025-00130

    Figure Lengend Snippet: CaSR mediated the osteogenic‐promoting effects of L‐Trp on MC3T3‐E1 cells. (A) Expression levels of Casr and its downstream genes (Gna11 and Pth1r). At 4 days, Casr expression was significantly higher in the L‐Trp‐treated group compared to the control group. By 7 days, the expression of Gna11 and Pth1r was also significantly elevated in the L‐Trp‐treated group. (B, C) Qualitative and semi‐quantitative analysis ( n = 3) of CASR‐related proteins. L‐Trp significantly upregulated the expression of CASR and GNA11. (D, E) Cell scratching experiment and the semi‐quantitative analysis. L‐Trp significantly enhanced the migration of MC3T3‐E1 cells. This effect was reversed by NPS‐2143, a CASR inhibitor, indicating that L‐Trp promoted cell migration through CASR activation. (F, G) ALP staining and alizarin red staining. L‐Trp significantly enhanced osteogenesis and the effect was attenuated by NPS‐2143, confirming that L‐Trp‐mediated osteogenic differentiation through CASR activation.

    Article Snippet: The primary antibodies included: CASR (1:500), GNA11 (1:1000), FAK (1:1000), RHOA (1:1000), Phospho‐FAK‐Y397 (1:2000), and Tubulin (1:5000) (All purchased from Abclonal, China).

    Techniques: Expressing, Control, Migration, Activation Assay, Staining